{"id":7869,"date":"2024-02-20T01:03:16","date_gmt":"2024-02-19T22:03:16","guid":{"rendered":"http:\/\/biotech.nahrainuniv.edu.iq\/?p=7869"},"modified":"2024-02-20T01:03:21","modified_gmt":"2024-02-19T22:03:21","slug":"%d9%85%d9%86%d8%a7%d9%82%d8%b4%d8%a9-%d8%b9%d9%84%d9%85%d9%8a%d8%a9-7","status":"publish","type":"post","link":"https:\/\/biotech.nahrainuniv.edu.iq\/?p=7869","title":{"rendered":"\u0645\u0646\u0627\u0642\u0634\u0629 \u0639\u0644\u0645\u064a\u0629"},"content":{"rendered":"\n

\u062a\u0645\u062a \u0645\u0646\u0627\u0642\u0634\u0629 \u0631\u0633\u0627\u0644\u0629 \u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0642\u0646\u064a\u0627\u062a \u0627\u0644\u0623\u062d\u064a\u0627\u0626\u064a\u0629 \u0628\u0639\u0646\u0648\u0627\u0646 “Cytotoxic potential of L-methioninase enzyme purified from clinical isolates of Pseudomonas spp” \u0642\u062f\u0645\u062a \u0627\u0644\u0631\u0633\u0627\u0644\u0629 \u0645\u0646 \u0642\u0628\u0644 \u0627\u0644\u0628\u0627\u062d\u062b\u0629 \u0622\u064a\u0629 \u0633\u0639\u062f\u0648\u0646 \u062f\u0627\u0648\u062f \u0628\u0627\u0634\u0631\u0627\u0641 \u0623.\u062f \u0631\u0642\u064a\u0629 \u0645\u062d\u0645\u062f \u0623\u0628\u0631\u0627\u0647\u064a\u0645 \u060c \u0647\u062f\u0641\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0625\u0644\u0649 \u062c\u0645\u0639 (150) \u0639\u064a\u0646\u0629 \u0645\u0631\u0636\u064a\u0629 \u0645\u0646 \u0645\u0635\u0627\u062f\u0631 \u0645\u062e\u062a\u0644\u0641\u0629 (\u0627\u0644\u062d\u0631\u0648\u0642, \u0627\u0644\u062c\u0631\u0648\u062d, \u0627\u0644\u062a\u0647\u0627\u0628\u0627\u062a \u0627\u0644\u0627\u0630\u0646, \u0627\u0644\u0628\u0644\u063a\u0645, \u0648\u0627\u0644\u062a\u0647\u0627\u0628\u0627\u062a \u0627\u0644\u0645\u062c\u0627\u0631\u064a \u0627\u0644\u0628\u0648\u0644\u064a\u0629) \u0644\u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0645\u0646 \u0645\u0633\u062a\u0634\u0641\u064a\u064a\u0646 \u062d\u0643\u0648\u0645\u064a\u064a\u0646. \u0648\u0628\u0646\u0627\u0621\u064b \u0639\u0644\u0649 \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0634\u062e\u064a\u0635 \u0627\u0644\u0645\u0638\u0647\u0631\u064a \u0648\u0627\u0644\u0643\u064a\u0645\u0648\u062d\u064a\u0648\u064a \u0642\u062f \u062a\u0628\u064a\u0646 \u0623\u0646 (33) \u0639\u0632\u0644\u0629 \u0645\u0646 \u0647\u0630\u0647 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0643\u0627\u0646\u062a \u0645\u0646 \u0646\u0648\u0639\u00a0 Pseudomonas spp . \u0643\u0645\u0627 \u062a\u0645 \u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0646\u0638\u0627\u0645 VITEK-2 \u0644\u062a\u0623\u0643\u064a\u062f \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0634\u062e\u064a\u0635. \u0627\u064f\u062e\u062a\u0628\u0631\u062a \u0642\u0627\u0628\u0644\u064a\u0629 \u0647\u0630\u0647 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0644\u0625\u0646\u062a\u0627\u062c \u0627\u0646\u0632\u064a\u0645 L- methioninase\u00a0 \u0648 \u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0623\u0646 (15) \u0639\u0632\u0644\u0629 \u0641\u0642\u0637 \u062a\u0646\u062a\u062c \u0627\u0646\u0632\u064a\u0645 MGL \u0628\u0645\u0633\u062a\u0648\u064a\u0627\u062a \u0645\u062e\u062a\u0644\u0641\u0629. \u0648\u0643\u0627\u0646\u062a \u0627\u0644\u0639\u0632\u0644\u0629\u00a0 \u00a0 \u00a0 \u00a0 P.aeruginosa A6 \u0623\u0639\u0644\u0649 \u0643\u0641\u0627\u0621\u0629 \u0641\u064a \u0627\u0644\u0625\u0646\u062a\u0627\u062c \u0648\u0644\u0630\u0644\u0643\u060c \u062a\u0645 \u0627\u062e\u062a\u064a\u0627\u0631 P. aeruginosa A6\u060c \u0645\u0646 \u0623\u062c\u0644 \u0625\u0646\u062a\u0627\u062c \u0648\u062a\u0646\u0642\u064a\u0629\u00a0 L- methioninase\u00a0 \u0641\u064a \u0627\u0644\u062e\u0637\u0648\u0629 \u0627\u0644\u0627\u062a\u064a\u0629 . \u0643\u0645\u0627 \u062a\u0636\u0645\u0646\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u0638\u0631\u0648\u0641 \u0627\u0644\u0645\u062b\u0644\u0649 \u0644\u0625\u0646\u062a\u0627\u062c \u0627\u0646\u0632\u064a\u0645 L- methioninase\u00a0 \u060c \u0648\u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0623\u0646 \u0643\u0645\u064a\u0629 L- methioninase\u00a0 \u0627\u0644\u0645\u0646\u062a\u062c\u0629 \u0645\u0646 \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627 \u062a\u0632\u062f\u0627\u062f \u0639\u0646\u062f \u0625\u0636\u0627\u0641\u0629 1% \u0633\u0643\u0631\u0648\u0632 \u06481% \u0643\u0627\u0632\u064a\u0646 (\u0643\u0645\u0635\u0627\u062f\u0631 \u0643\u0631\u0628\u0648\u0646 \u0648\u0646\u064a\u062a\u0631\u0648\u062c\u064a\u0646 \u0639\u0644\u0649 \u0627\u0644\u062a\u0648\u0627\u0644\u064a) \u0627\u0644\u0649 \u0627\u0644\u0648\u0633\u0637M9 \u0627\u0644\u0645\u0639\u062f\u0644 \u0645\u0639 \u0645\u0633\u062a\u0648\u0649 \u062d\u0627\u0645\u0636\u064a 7.0pH=. \u0648\u0644\u0641\u062a\u0631\u0629 \u062d\u0636\u0646 \u0644\u0645\u062f\u0629 48 \u0633\u0627\u0639\u0629 \u0639\u0646\u062f \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 37 \u062f\u0631\u062c\u0629 \u0645\u0626\u0648\u064a\u0629. \u062a\u0636\u0645\u0646\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u064a\u0636\u0627\u064b\u060c \u062a\u0646\u0642\u064a\u0629 L-methioninase \u0641\u064a \u062b\u0644\u0627\u062b \u062e\u0637\u0648\u0627\u062a \u0634\u0645\u0644\u062a\u061b \u0623\u0648\u0644\u0627\u064b \u0627\u0644\u062a\u0631\u0633\u064a\u0628 \u0628\u062a\u0634\u0628\u0639 \u0643\u0628\u0631\u064a\u062a\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0628\u0645\u0639\u062f\u0644\u00a0 70% \u060c \u062b\u0627\u0646\u064a\u0627\u064b \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u062c\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u062a\u0628\u0627\u062f\u0644 \u0627\u0644\u0623\u064a\u0648\u0646\u064a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u0645\u0648\u062f DEAE-Cellulose\u060c \u0628\u064a\u0646\u0645\u0627 \u0643\u0627\u0646 \u0639\u0645\u0648\u062f \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u062c\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u062a\u0631\u0634\u064a\u062d \u0627\u0644\u0647\u0644\u0627\u0645\u064a Sephadex G-150 \u0647\u0648 \u0627\u0644\u062e\u0637\u0648\u0629 \u0627\u0644\u0623\u062e\u064a\u0631\u0629. \u062d\u064a\u062b \u0632\u0627\u062f \u0627\u0644\u0646\u0634\u0627\u0637 \u0627\u0644\u0645\u062d\u062f\u062f \u0644\u0640 \u00a0 MGL\u00a0 \u0627\u0644\u0645\u0646\u0642\u0649 \u0625\u0644\u0649 18.6 \u0648\u062d\u062f\u0629\/\u0645\u062c\u0645 \u0645\u0646 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u060c \u0628\u0639\u062f \u062a\u0646\u0642\u064a\u062a\u0647 \u0628\u0627\u0644\u062a\u0631\u0634\u064a\u062d \u0627\u0644\u0647\u0644\u0627\u0645\u064a. \u0643\u0645\u0627 \u062a\u0645 \u062a\u062d\u062f\u064a\u062f \u0627\u0644\u0648\u0632\u0646 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0644\u0640 L-methioninase \u0627\u0644\u0645\u0646\u0642\u0649 \u0648\u0642\u062f \u0648\u062c\u062f \u0628\u0623\u0646\u0647 \u064a\u0633\u0627\u0648\u064a 55 \u0643\u064a\u0644\u0648 \u062f\u0627\u0644\u062a\u0648\u0646 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 SDS-PAGE . \u0627\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0633\u0645\u064a \u0627\u0644\u062e\u0644\u0648\u064a \u0644\u0627\u0646\u0632\u064a\u0645MGL\u00a0 \u0639\u0644\u0649 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629\u060c \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0631\u0627\u0643\u064a\u0632 \u0645\u062e\u062a\u0644\u0641\u0629 (25-400 \u0645\u064a\u0643\u0631\u0648\u062c\u0631\u0627\u0645\/\u0645\u0644) \u0645\u0646 L- methioninase\u00a0 \u0639\u0644\u0649 \u062e\u0637\u0648\u0637 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0627\u0644\u0643\u0628\u062f\u064a\u0629 HepG2 \u0648\u0627\u0644\u0642\u0648\u0644\u0648\u0646 CaCo-2\u00a0 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u062e\u062a\u0628\u0627\u0631 .MTT \u0648\u0642\u062f \u0627\u0648\u0636\u062d\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0646 \u0627\u0646\u0632\u064a\u0645 L- methioninase\u00a0 \u0644\u0647 \u0627\u0644\u0642\u0627\u0628\u0644\u064a\u0629 \u0639\u0644\u0649 \u062a\u062b\u0628\u064a\u0637 \u0646\u0645\u0648 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0627\u0644\u062d\u064a\u0629 \u0628\u0627\u0644\u0627\u0639\u062a\u0645\u0627\u062f \u0639\u0644\u0649 \u0627\u0644\u062a\u0631\u0643\u064a\u0632. \u0648\u0642\u062f \u0627\u0645\u062a\u0644\u0643 \u0627\u0644\u0642\u0627\u0628\u0644\u064a\u0629 \u0639\u0644\u0649 \u062a\u062b\u0628\u064a\u0637 \u0646\u0645\u0648 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0643\u0628\u062f \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0627\u0644\u062d\u064a\u0629 HepG2 \u0644\u0640MGL \u0627\u0644\u062e\u0627\u0645 \u0645\u0639 IC50\u00a0 \u0642\u062f\u0631\u0647 140.0 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644 \u0628\u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 IC50 67.44 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644 \u0644\u0644\u0625\u0646\u0632\u064a\u0645 \u0627\u0644\u0645\u0646\u0642\u0649. \u0628\u064a\u0646\u0645\u0627 \u0627\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0644\u0627\u0646\u0632\u064a\u0645 L-methioninase \u0627\u0644\u062e\u0627\u0645 \u0642\u062f\u0631\u062a\u0647 \u0639\u0644\u0649 \u062a\u062b\u0628\u064a\u0637 \u0646\u0645\u0648 \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0642\u0648\u0644\u0648\u0646 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0645\u0639 IC50 \u064a\u0628\u0644\u063a 154.3 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644 \u0628\u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 IC50 20.57 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644 \u0644\u0644\u0625\u0646\u0632\u064a\u0645 \u0627\u0644\u0645\u0646\u0642\u0649. \u0643\u0645\u0627 \u0627\u0644\u062c\u062f\u064a\u0631 \u0628\u0627\u0644\u0630\u0643\u0631\u060c \u0623\u0646 \u062e\u0637 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 HdFn \u0644\u0645 \u064a\u062a\u0623\u062b\u0631 \u0628\u0634\u0643\u0644 \u0643\u0628\u064a\u0631 \u0628\u0627\u0644\u0625\u0646\u0632\u064a\u0645. \u0623\u0638\u0647\u0631\u062a \u062c\u0645\u064a\u0639 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0645\u0630\u0643\u0648\u0631\u0629 \u0623\u0639\u0644\u0627\u0647, \u0623\u0646 \u0647\u0646\u0627\u0644\u0643 \u0646\u0634\u0627\u0637 \u0633\u0645\u064a \u062e\u0644\u0648\u064a \u0644\u0623\u0646\u0632\u064a\u0645 L-methioninase \u0627\u0644\u0645\u0646\u0642\u0649 \u0648\u0627\u0644\u062e\u0627\u0645 \u0627\u0644\u0645\u0639\u0632\u0648\u0644 \u0645\u0646 P. aeruginosa \u0628\u0623\u062a\u0628\u0627\u0639 \u0637\u0631\u0642 \u062a\u0646\u0642\u064a\u0629 \u0645\u062e\u062a\u0644\u0641\u0629.<\/p>\n\n\n\n

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\"\"<\/a><\/figure>\n\n\n\n
\"\"<\/a><\/figure>\n\n\n\n
\"\"<\/a><\/figure>\n\n\n\n
\"\"<\/a><\/figure>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"

\u062a\u0645\u062a \u0645\u0646\u0627\u0642\u0634\u0629 \u0631\u0633\u0627\u0644\u0629 \u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0642\u0646\u064a\u0627\u062a \u0627\u0644\u0623\u062d\u064a\u0627\u0626\u064a\u0629 \u0628\u0639\u0646\u0648\u0627\u0646 “Cytotoxic potential of L-methioninase enzyme purified from clinical isolates of Pseudomonas spp” \u0642\u062f\u0645\u062a \u0627\u0644\u0631\u0633\u0627\u0644\u0629 \u0645\u0646 \u0642\u0628\u0644 \u0627\u0644\u0628\u0627\u062d\u062b\u0629 \u0622\u064a\u0629 \u0633\u0639\u062f\u0648\u0646 \u062f\u0627\u0648\u062f \u0628\u0627\u0634\u0631\u0627\u0641 \u0623.\u062f \u0631\u0642\u064a\u0629 \u0645\u062d\u0645\u062f \u0623\u0628\u0631\u0627\u0647\u064a\u0645 \u060c \u0647\u062f\u0641\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0625\u0644\u0649 \u062c\u0645\u0639 (150) \u0639\u064a\u0646\u0629 \u0645\u0631\u0636\u064a\u0629 \u0645\u0646 \u0645\u0635\u0627\u062f\u0631 \u0645\u062e\u062a\u0644\u0641\u0629 (\u0627\u0644\u062d\u0631\u0648\u0642, \u0627\u0644\u062c\u0631\u0648\u062d, \u0627\u0644\u062a\u0647\u0627\u0628\u0627\u062a \u0627\u0644\u0627\u0630\u0646, \u0627\u0644\u0628\u0644\u063a\u0645, \u0648\u0627\u0644\u062a\u0647\u0627\u0628\u0627\u062a \u0627\u0644\u0645\u062c\u0627\u0631\u064a \u0627\u0644\u0628\u0648\u0644\u064a\u0629) \u0644\u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 … <\/p>\n

Continue reading “\u0645\u0646\u0627\u0642\u0634\u0629 \u0639\u0644\u0645\u064a\u0629”<\/span><\/a><\/p>\n","protected":false},"author":1113,"featured_media":7873,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[27,42,44,49],"tags":[],"class_list":["post-7869","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized-ar","category-42","category-44","category-49","entry"],"_links":{"self":[{"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/7869","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=\/wp\/v2\/users\/1113"}],"replies":[{"embeddable":true,"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7869"}],"version-history":[{"count":1,"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/7869\/revisions"}],"predecessor-version":[{"id":7874,"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/7869\/revisions\/7874"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=\/wp\/v2\/media\/7873"}],"wp:attachment":[{"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7869"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7869"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biotech.nahrainuniv.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7869"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}